论文部分内容阅读
研究观察了Ｍ－ＣＳＦ和ＩＦＮ－γ基因转染的巨噬细胞（Ｍａｃｒｏｐｈａｇｅ，Ｍφ）的体外抗原提呈能力，并对其机制进行了初步探讨，结果表明，ＩＦＮ－γ基因转染及Ｍ－ＣＳＦ／ＩＦＮ－γ联合基因转染的Ｍφ抗原提呈功能显著增强，联合基因转染组优于单基因转染组（Ｐ＜０．０５），Ｍφ表面Ｉａ、Ｂ７－１、ＩＣＡＭ－１的表达水平有不同程度的提高，这些细胞表面相关分子的高表达可能与其抗原提呈能力增加有关。混合淋巴细胞反应结果提示，当用５倍于Ｍφ量的肿瘤细胞制备的肿瘤抗原触发４ｈ，Ｔ淋巴细胞的增殖能力最强，该结果为过继回输肿瘤抗原体外触发的基因转染的Ｍφ治疗肿瘤提供了实验依据。
To study the in vitro antigen-presenting ability of M-CSF and IFN-γ-transfected macrophages (Macrophage, Mφ), and to explore its mechanism. The results showed that IFN-γ gene transfection and M- (P <0.05). The expression of Mφ antigen on the surface of Mφ on the surface of Mφ was significantly higher than that on the Mφ The level of expression increased to varying degrees, the high expression of these cell surface molecules may be related to its increased antigen presenting capacity. The result of mixed lymphocyte reaction suggested that T lymphocytes had the strongest proliferative ability when tumor antigen prepared by using 5 times the amount of Mφ tumor cells was activated for 4h. This result was Mφ treatment of gene transfection triggered by adoptive transfer of tumor antigen in vitro Tumor provides an experimental basis.