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目的探讨红藻氨酸(Kainic Acid,KA)致癫痫大鼠癫痫发作过程中海马组织神经生长因子(nerve growth factor,NGF)不同时间点表达量的变化。方法腹腔注射KA建立大鼠癫痫模型。采用Taq Man探针实时定量PCR(Real-time quantitative PCR)技术,检测注射KA后不同时点大鼠海马组织中NGF表达量的变化。结果与NGF表达量与生理盐水对照组(NS)相比,6~12 h NGF表达量明显低于NS组(P<0.05)、48~72 h NGF表达量开始升高并高于NS组,在24、72 h时其表达量显著高于NS组(P<0.05)。结论 NGF对致癫大鼠的海马具有修复与保护作用。
Objective To investigate the changes of the expression of nerve growth factor (NGF) in hippocampus of rats with epilepsy induced by Kainic Acid (KA). Methods The model of epilepsy in rats was established by intraperitoneal injection of KA. TaqMan probe real-time quantitative PCR (Real-time quantitative PCR) technology was used to detect the changes of NGF expression in hippocampal tissue of rats at different time points after injection of KA. Results Compared with the NS group, the expression of NGF was significantly lower than that of the NS group (P <0.05), and the expression of NGF began to increase from 48 to 72 h and was higher than that of the NS group At 24 and 72 h, the expression level was significantly higher than that in NS group (P <0.05). Conclusion NGF can repair and protect the hippocampus of epileptic rats.