用套式ＰＣＲ（ｎｅｓｔｅｄＰＣＲ）对广西１６例原发性肝癌患者血清ＨＢＶＤＮＡ核心基因启动子进行扩增，阳性者用Ｓａｎｇｅｒ氏ＤＮＡ测序法进行序列分析。结果表明１４例患者ＰＣＲ阳性，阳性率为８７．５０％，其中除唯一的ＨＢｅＡｇ阳性者（Ｃ２３）外，均具有双突变（即第１７６２位Ａ→Ｔ，１７６４位Ｇ→Ｔ核苷酸的点突变），而且所有的血清标本在这突变点周围均有不同部位的点突变，但在标本Ｃ２３整个启动子中，未发现任何碱基突变。本文对这种突变株可能的致癌机理进行了讨论。 Nested PCR was used to amplify the HBV DNA core gene promoter in 16 patients with primary liver cancer in Guangxi. The positive samples were sequenced using Sanger’s DNA sequencing method. The results showed that 14 patients had positive PCR, the positive rate was 87.50%, except for the only HBeAg positive (C23), all had double mutations (ie, 1762 A→T, 1764 G→T nucleotides Point mutations), and all serum samples had point mutations at different sites around the mutation, but no mutations were found in the entire promoter of sample C23. This article discusses the possible carcinogenic mechanisms of this mutant strain.