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目的:建立同时测定抗骨质疏松复方制剂中淫羊藿苷、川续断皂苷Ⅵ、蛇床子素的高效液相色谱法。方法:采用C_(18)柱,流动相为乙腈-水梯度洗脱,检测波长为205nm,流速为1mL·min~(-1);柱温为30℃。结果:在该色谱条件下,淫羊藿苷,川续断皂苷Ⅵ及蛇床子素能同时检出,专属性好,线性回收率、精密度等均符合要求。结论:该方法简便、快速、灵敏、重现性好,能同时准确测定3种活性成分,可供该制剂活性成分含量测定用。
Objective: To establish a simultaneous determination of anti-osteoporosis compound icariin, Dictyostellaria Ⅵ, osthole by high performance liquid chromatography. Methods: The mobile phase consisted of C18 column with gradient elution of acetonitrile and water. The detection wavelength was 205 nm and the flow rate was 1 mL · min ~ (-1). The column temperature was 30 ℃. Results: Under the chromatographic conditions, icariin, Dictyosaponin Ⅵ and osthole were detected simultaneously with good specificity, linear recovery, precision and so on. Conclusion: The method is simple, rapid, sensitive and reproducible. It can simultaneously determine the three active ingredients for the determination of the active ingredient content of the preparation.