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目的:探索HPLC测定舒胸脉冲控释滴丸中阿魏酸和羟基红花黄色素A的含量。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.01 mol.L-1磷酸二氢钾水溶液(22∶78)用磷酸调节pH 3.0为流动相,流速1.0 mL.min-1,检测波长403,322 nm,柱温30℃。结果:阿魏酸在1.409～90.16 mg.L-1回归方程为A=0.820 6 C-0.287 4(r=0.9999),平均回收率为99.43%,RSD 3.42%(n=6);羟基红花黄色素A在4.035～258.3 mg.L-1,回归方程为A=0.412 7C-0.487 8(r=0.999 7),平均回收率为100.03%,RSD 3.55%(n=6)。结论:该方法操作简便,准确,重复性良好,可用于舒胸脉冲控释滴丸的质量控制。
Objective: To explore the content of ferulic acid and hydroxysafflor yellow A in Shu-pulsatile controlled-release drip pills by HPLC. METHODS: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used. Phosphate was added to the solution to adjust the pH value to 3.0 with a methanol-0.01 mol·L-1 aqueous solution of potassium dihydrogen phosphate (22:78) at a flow rate of 1.0 mL · min -1, detection wavelength 403,322 nm, column temperature 30 ℃. Results: The regression equation of ferulic acid at 1.409 ~ 90.16 mg.L-1 was A = 0.820 6 C-0.287 4 (r = 0.9999) with the average recovery of 99.43% and RSD of 3.42% (n = 6) The average recovery was 100.03% and the RSD was 3.55% (n = 6) for the yellow pigment A at 4.035-258.3 mg.L-1. The regression equation was A = 0.412 7C-0.487 8 (r = 0.999 7). Conclusion: The method is simple, accurate and reproducible. It can be used to control the quality of pulsatile controlled release pills.