论文部分内容阅读
目的制作侧颅底火棉胶水平位断层连续薄层切片,获取数字化图片库,为影像学断层研究及侧颅底疾病临床诊断提供参考。方法用2例尸头标本进行螺旋CT扫描后制作侧颅底区标本。火棉胶包埋后,用德国产大型轮式切片机沿水平位切片,厚度100μm,每切一片均采用Sony F-717数码照相机(500万像素)摄影并保存在计算机。将断层照片与HE染色照片和CT影像进行对照观察,选取代表性层面进行描述,包括外半规管层面,面神经水平段层面,前庭窗层面,圆窗层面。结果共获取数字化连续图片2套,分别为300、340张,断层图像分辨率1920×2560像素,结构毗邻关系显示清晰。与HE照片和CT片对照,可以更清楚地定位断层结构。结论火棉胶包埋技术是制作侧颅底大切片,获取高分辨率的数字化图片的理想方法,对颞骨内解剖结构的毗邻关系显示清晰,组织学与CT对照观察是研究侧颅底水平位断层解剖特点的重要方法,对指导影像学诊断具有重要参考价值。
OBJECTIVE: To make a continuous slice of slices of horizontal cutaneous flagellate at the side of the skull and obtain a digital image database to provide reference for the study of imaging and the clinical diagnosis of skull base diseases. Methods Two cases of cadaver heads were used for spiral CT scan to make specimens of lateral cranial base. After the fire cotton glue is embedded, it is sliced along a horizontal plane with a large German wheeled microtome with a thickness of 100 μm. Each piece is photographed with a Sony F-717 digital camera (5 million pixels) and stored in a computer. The tomographic images were compared with HE-stained photographs and CT images. The representative layers were selected for the description, including the lateral semicircular canal surface, facial nerve level segment, the vestibular window level and the round window level. Results A total of 2 sets of digitalized continuous pictures were obtained, which were 300,340 and the resolution of the tomographic image was 1920 × 2560 pixels respectively. The adjacent structures were clearly displayed. In contrast to HE and CT images, the tomographic structure can be more clearly located. Conclusion collodion embedding technology is making big slice lateral skull base, the ideal way to high-resolution digital images of the acquisition of adjacent structures within the temporal bone anatomy showing a clear, histology and CT control observation is the level of research lateral skull base position An important method of anatomic features of the tomography, the guidance of diagnostic imaging has an important reference value.