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作者对体外培养的人牙龈成纤维细胞(GF)和牙周韧带细胞(PDLC)采用免疫组化ABC染色及显微分光光度法分析,测定GF和PDLC中前列腺素E2(PGE2)、6-酮一前列腺素F1a.(6-K-PGF1a)和血栓素B2(TXB2)含量,以探讨其与牙周炎的关系,结果表明GF的PGE2吸光度为0.25±0.03,6-K-PGF1a的吸光度为0.20±0.03,与对照组相比均有非常显著性差异;PDLC的吸光度分别为PGE20.21±0.02、TXB20.16±0.03和6-K-PGF1a0.13±0.02,与对照组相比均有非常显著性差异。表明GF和PDLC均可分泌一定量的PGE2、TXB2和6-K-PGF1a,但分泌的量略有差异,提示GF和PDLC可通过分泌PG,影响牙龈炎症变化和牙周组织再生。
In this study, the human gingival fibroblasts (GFs) and periodontal ligament cells (PDLCs) cultured in vitro were stained with ABC and analyzed by microdispectrophotometry to determine the contents of prostaglandin E2 (PGE2) and 6-keto Prostaglandin F1a. (6-K-PGF1a) and thromboxane B2 (TXB2) content in order to investigate its relationship with periodontitis. The results showed that the absorbance of PGF2 of GF was 0.25 ± 0.03, and the absorbance of K-PGF1a was 0 .20 ± 0.03, compared with the control group, there was a very significant difference; PDLC absorbance were PGE20.21 ± 0.02, TXB20.16 ± 0.03 and 6-K-PGF1a0.13 ± 0. 02, compared with the control group have very significant differences. Both GF and PDLC can secrete a certain amount of PGE2, TXB2 and 6-K-PGF1a, but the secretion amount is slightly different, suggesting that GF and PDLC can affect the gingival inflammation and periodontal tissue regeneration by secreting PG.