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目的 探讨抗ＣＤ３ 单克隆抗体（ＡｎｔｉＣＤ３ＭｃＡｂ）＋ 重组人白细胞介素２（ｒＩＬ２） 激活的脐血杀伤细胞（ＣＤ３ＡＫ） 细胞对白血病细胞株Ｋ５６２ 、ＨＬ６０ 的杀伤作用。方法 采用间接玫瑰花结法测定脐血单个核细胞表面分化抗原，ＥＬＩＳＡ双抗夹心法测定肿瘤坏死因子α（ＴＮＦα）、ＩＬ６ 和ＩＬ８ ，用β液闪仪测定３Ｈ胸腺嘧啶核苷标记的靶细胞杀伤活性。结果 ①经ＡｎｔｉＣＤ３ＭｃＡｂ＋ ｒＩＬ２ 激活的脐血单个核细胞免疫表型发生明显变化；②细胞因子水平较未激活组明显增高；③脐血ＣＤ３ＡＫ 细胞对Ｋ５６２、ＨＬ６０细胞均有杀伤作用，最佳杀伤条件：脐血单个核细胞浓度为１ ×１０６／ｍｌ，ＡｎｔｉＣＤ３ＭｃＡｂ １ μｇ／ｍｌ，ｒＩＬ２ １０００ Ｕ／ｍｌ，作用时间７２ 小时，效靶比１００∶１。结论 脐血单个核细胞能被ＡｎｔｉＣＤ３ ＭｃＡｂ＋ ｒＩＬ２ 激活为杀伤细胞，激活后有较强的细胞毒作用，且对白血病细胞株有明显的杀伤作用，该研究为应用活化脐血进行肿瘤的过继免疫治疗提供理论依据。
Objective To investigate the killing effects of anti-CD3 monoclonal antibody (AntiCD3McAb) + recombinant human interleukin 2 (rIL2) activated cord blood killer (CD3AK) cells on leukemia cell lines K562 and HL60. Methods Indirect rosette method was used to determine the surface differentiation antigen of umbilical cord blood mononuclear cells. TNF-α, IL-6 and IL-8 were measured by ELISA with double antibody sandwich method. Thymine labeled target cell killing activity. Results 1 The immunophenotype of cord blood mononuclear cells activated by AntiCD3McAb+ rIL2 changed significantly; 2 The level of cytokines was significantly higher than that of the unactivated group; 3 CD3AK cells of cord blood had killing effect on K562 and HL60 cells. , The best killing conditions: umbilical cord blood mononuclear cell concentration of 1 × 106/ml, Anti CD3McAb 1 μg/ml, rIL 2 1000 U/ml, the role of time 72 hours, the effective target ratio of 100:1. Conclusion Umbilical cord blood mononuclear cells can be activated by anti-CD3 McAb+ rIL2 as killer cells. After activation, they have strong cytotoxicity and have a significant killing effect on leukemia cell lines. This study uses activated cord blood to perform tumors. The use of adoptive immunotherapy provides a theoretical basis.