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目的测定三叶因子1在正常及病理条件下胃粘膜中的表达情况,探讨TFF1在胃粘膜损伤修复及胃癌抑制中的作用及意义。方法应用免疫组化方法测定正常及不同病理条件下胃粘膜中TFF1的表达情况,通过图像分析软件分析其阳性信号平均光密度值了解其表达情况。结果胃炎、胃溃疡及十二指肠球部溃疡患者TFF1表达明显高于正常胃粘膜;多发/复合性溃疡患者TFF1表达高于单发的消化性溃疡患者;胃溃疡患者与十二指肠球部溃疡患者、胃炎患者与单一消化性溃疡患者表达相比均无统计学差异;胃腺癌患者癌旁组织表达明显高于正常胃粘膜,而腺癌组织的表达强度则与癌组织的分化程度呈正比,分化程度愈低,表达愈弱,低分化腺癌无阳性表达,中、高分化腺癌表达略低于正常粘膜,但二者对比无统计学差异;食管鳞癌及癌旁组织均无阳性表达;各组男女对比均无统计学差异。结论TFF1在胃炎及消化性溃疡中表达升高,且在多发/复合性溃疡中的表达较单发溃疡表达升高,即粘膜损伤越严重其表达越强,提示其在胃粘膜保护及促进上皮重建机制中具有一定的作用。TFF1在癌旁组织中表达增强提示其可能与肿瘤抑制及分化机制有关,而在癌组织中表达减弱,且分化程度越低表达越弱可能与癌组织腺体及细胞破坏、TFF1的分泌减少有关。
Objective To determine the expression of trefoil factor 1 in gastric mucosa under normal and pathological conditions and to explore the role and significance of TFF1 in gastric mucosal lesion repair and gastric cancer. Methods Immunohistochemical method was used to determine the expression of TFF1 in gastric mucosa under normal and different pathological conditions. The expression of TFF1 was analyzed by image analysis software. Results The expression of TFF1 in patients with gastritis, gastric ulcer and duodenal ulcer was significantly higher than that in normal gastric mucosa. The expression of TFF1 in patients with multiple ulcer / multiple ulcer was higher than that in patients with peptic ulcer. The patients with gastric ulcer and duodenal bulb There was no significant difference in the expression of paracancerous tissue between gastric ulcer patients and gastritis patients compared with the single peptic ulcer patients. The expression of paracancerous tissues in patients with gastric adenocarcinoma was significantly higher than that in normal gastric mucosa, while the expression intensity in adenocarcinoma tissues was positively correlated with the differentiation degree The lower the degree of differentiation, the weaker the expression, the poorly differentiated adenocarcinoma had no positive expression in moderate and well-differentiated adenocarcinoma, but the difference between them was not statistically significant. There was no significant difference between esophageal squamous cell carcinoma and para-cancerous tissue Positive expression; no significant difference between men and women in each group. Conclusion The expression of TFF1 in gastritis and peptic ulcer is increased, and the expression of TFF1 is higher in multiple / complex ulcer than that in single ulcer, which means the more severe the damage of mucosa is, the stronger its expression is, suggesting that TFF1 can protect and promote the epithelium Reconstruction mechanism has a certain role. The increased expression of TFF1 in paracancerous tissues suggests that the expression of TFF1 may be related to the mechanism of tumor suppression and differentiation, while the expression of TFF1 is weakened in cancerous tissues. The weakened differentiation may be related to the destruction of glandular tissue and cells and the decrease of TFF1 secretion .