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报道了一种测定水溶液中核酸的方法，该法基于核酸对氯化银溶胶共振散射光的猝灭作用。在理想测定条件下，散射光的猝灭程度正比于核酸的浓度，三种核酸（ｃａｌｆ ｔｈｙｍｕｓrrrrrrrrrn ＤＮＡ， ｈｅｒｒｉｎｇ ＤＮＡ ａｎｄ Ｙｅａｓｔ ＲＮＡ）的线性范围分别为 ０－ ２０μｇ／Ｌ，０～ ６０μｇ／Ｌ和 ０～ ８０μｇ／Ｌ，检 测限分别为 ０．６５ μｇ／Ｌ，１．１μｇ／Ｌ和 １．９ μｇ／Ｌ。 ６种合成样品的测定结果令人满意。机理研究结果表明，核酸中的碱基（尤其是嘌呤碱）同银离子具有很强的结合能力，这种结合影响了氯化银的沉淀平衡，导致了氯化银溶胶共振散射光的猝灭。
A method for the determination of nucleic acids in aqueous solution based on the quenching effect of nucleic acids on the scattered light of silver chloride sols is reported. Under ideal conditions, the degree of quenching of the scattered light is proportional to the concentration of the nucleic acid. The three nucleic acids (calf thymus r r r r r r n DNA, herring DNA and Yeast The linear range was 0-20μg / L, 0-60μg / L and 0-80μg / L, respectively. The detection limits were 0.65μg / L, 1.1μg / L and 1.9μg / L, respectively. The results of six synthetic samples were satisfactory. The results of the mechanism study show that the base of the nucleic acid (especially the purine base) has strong binding ability with the silver ions, and this combination affects the precipitation balance of the silver chloride and leads to the quenching of the silver chloride sol by the scattered light .