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目的 研究端粒酶活性及c myc、p16在胃黏膜癌变过程中的作用。 方法 应用PCR -ELISA法检测 171例胃癌及相关组织中端粒酶活性 ;免疫组织化学法检测c myc、p16蛋白表达状况。并分析它们之间的相关性。 结果 胃癌组织端粒酶活性和c myc蛋白的表达率均最高 ,p16蛋白表达率最低 ;慢性浅表性胃炎组织 p16蛋白表达率最高。慢性萎缩性胃炎伴中、重度肠化组织中 ,端粒酶阳性和阴性组c myc和 p16蛋白表达率相互比较均有显著性差异 (P <0 .0 5 ) ;胃癌组织中 ,端粒酶阳性和阴性组c myc蛋白表达率有非常显著性差异 (P <0 .0 1) ;p16的表达率无显著性差异 (P >0 .0 5 )。 结论 在慢性萎缩性胃炎伴中、重度肠化组织中端粒酶活性和c myc的高表达及 p16的失活具有协同性 ,c myc的激活 ,p16的失活可能是端粒酶活化的 1个必要条件。
Objective To study the role of telomerase activity and c myc and p16 in carcinogenesis of gastric mucosa. Methods The telomerase activity was detected in 171 cases of gastric cancer and related tissues by PCR-ELISA. The expression of c myc and p16 protein was detected by immunohistochemistry. And analyze the correlation between them. Results The telomerase activity and c myc protein expression rate were highest in gastric cancer tissues, and the p16 protein expression rate was the lowest. The expression rate of p16 protein in chronic superficial gastritis tissues was the highest. In chronic atrophic gastritis with moderate to severe intestinal metaplasia, the expression rates of c myc and p16 proteins in the positive and negative telomerase groups were significantly different from each other (P < 0.05); in gastric cancer tissues, telomerase There was a significant difference in the expression of c myc protein between positive and negative groups (P < 0.01); there was no significant difference in the expression of p16 (P > 0.05). Conclusions Telomerase activity and high expression of c myc in chronic atrophic gastritis with moderate and severe intestinal metaplasia and p16 inactivation are synergistic, c myc activation, p16 inactivation may be telomerase activation 1 Necessary conditions.