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目的:评价牙本质蛋白聚糖对脱矿牙本质胶原纤维形貌和水合性能的影响。方法:新鲜拔除无龋坏人磨牙牙本质酸蚀后分别用胰蛋白酶和硫酸软骨素酶ABC孵育去除牙本质蛋白聚糖和糖胺聚糖侧链,对照组与实验组处理方法相同,但孵育液中不添加酶。然后在牙本质表面不同润湿状态下用场发射扫描电镜和激光共聚焦扫描电镜分别观察牙本质的微观形貌并评价脱矿牙本质的水合性能。结果:硫酸软骨素酶ABC和TRY酶处理改变了牙本质的微观形貌,使胶原纤维间距增大。酶处理、牙本质表面润湿性及两者的交互作用均会显著影响脱矿牙本质的厚度(P<0.0001)。结论:牙本质蛋白聚糖和糖胺聚糖侧链在维持牙本质胶原纤维网的空间结构和水合作用方面均发挥着重要作用。蛋白聚糖、胶原纤维-蛋白聚糖以及蛋白聚糖-蛋白聚糖间的的亲水性是影响脱矿牙本质围观形貌和厚度的重要因素。
Objective: To evaluate the effect of dentin proteoglycan on morphologies and hydration properties of demineralized dentin collagen fibers. METHODS: Freshly extracted tooth carnallin was extracted from the toothless dentine and removed by trypsin and chondroitinase ABC respectively to remove the dentin proteoglycan and glycosaminoglycan side chains. The control group and the experimental group were treated in the same way but incubated No enzyme added to the solution. Then the dentin surface under different wetting conditions using field emission scanning electron microscopy and confocal laser scanning microscopy were observed dentin micromorphology and evaluation of demineralized dentine hydration performance. Results: The treatment of chondroitinase ABC and TRY changed the micromorphology of dentin and increased the distance between collagen fibers. Enzyme treatment, dentin surface wettability and their interaction both significantly affected the thickness of demineralized dentin (P <0.0001). CONCLUSION: The dentin proteoglycan and glycosaminoglycan side chains play an important role in maintaining the spatial structure and hydration of dentin collagen network. Hydrophilicity between proteoglycan, collagen fiber-proteoglycan and proteoglycan-proteoglycans is an important factor affecting the topography and thickness of demineralized dentin.