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Objective:To identify inhibitors of nitric oxide production and NF-κB activity from Chromolaena odorata(C.odorata).Methods:The compounds isolated from the aerial parts of C.odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-κB activity in LPS-stimulated RAW264.7 cells.Results:Six fatty acids (S)-coriolic acid(1),(S)-coriolic acid methyl ester(2),(5)-15,16-didehydrocoriolic acid(3),(S)- 15,16-didehydrocoriolic acid methyl ester(4),linoleamide(5) and linolenamide(6) were isolated. All compounds inhibited the NO production at concentrations consistent with those required for NF-κB inhibition.Compound 2 was the most active with the IC_(50) values of 5.22 and 5.73μM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities.Conclusions:The fatty acid components in C.odorata with NF-κB inhibitory activity could explain the anti-inflammation property of this plant in traditional medicine.This study could also contribute to the better use of C.odorata for human health care.
Objective: To identify inhibitors of nitric oxide production and NF-κB activity from Chromolaena odorata (C. odorata). Methods: The compounds isolated from the aerial parts of C. odorata by bioassay-guided fractionation were investigated for their inhibitory effects on the NO production and NF-κB activity in LPS-stimulated RAW264.7 cells. Results: Six fatty acids (S) -coriolic acid (1), (S) -coriolic acid methyl ester (2), All compounds inhibited the NO production at concentrations consistent with those required for NF-κB (3), (S) -15,16-didehydrocoriolic acid methyl ester (4), linoleamide (5) and linolenamide inhibition. Compound 2 was the most active with the IC 50 values of 5.22 and 5.73 μM. The addition of a double bond in the fatty chain decreased the inhibitory effects while the methyl esterification increased the activities. Conclusions: The fatty acid components in C. odorata with NF-κB inhibitory activity could explain the anti-inflammation pr operty of this plant in traditional medicine.This study could also contribute to the better use of C. odorata for human health care.