为研究肺炎衣原体与分泌性中耳炎的关系以115例分泌性中耳炎患者为研究对象,其中急性(或亚急性)分泌性中耳炎73例,年龄2～77岁,平均40岁;病程3个月以上慢性者42例,年龄3～71岁,平均27岁。切开鼓膜,吸引中耳液,用细的棉棒采取标本,当日或次日行细胞接种。72小时培养后行吉姆萨染色,采用单克隆抗体的荧光抗体法测定肺炎衣原体。同 In order to study the relationship between Chlamydia pneumoniae and secretory otitis media, 115 patients with secretory otitis media were studied, including 73 cases of acute (or subacute) otitis media with effusion, aged from 2 to 77 years, average 40 years old. In 42 cases, aged 3 to 71 years, mean 27 years. Cut the tympanic membrane, to attract the middle ear fluid, with a thin cotton swab to take specimens, the day or next day line cell inoculation. After 72 hours of culture, Giemsa staining was performed. Chlamydia pneumoniae was detected by the fluorescent antibody method of monoclonal antibody. with