用构建好的乙肝病毒基因亚克隆ｐＢａｍＨＩＨｉｎｃｌＣＡＴ２转染人特异性肝癌细胞ＨｅｐＧ２及人非特异性肝癌细胞Ｈｅｌａ，通过相应配体的刺激，使细胞内氯霉素乙酰基转移酶活力明显增强．进一步的体外蛋白阻滞分析，确定在该亚克隆上含有一新的维甲素受体反应元件．此元件序列极端保守，由相隔６个碱基的重复序列构成 HepG2 and human non-specific hepatoma Hela cells were transfected with the constructed sub-clone of pBamHIHinclCAT2, and the activity of chloramphenicol acetyltransferase in the cells was significantly enhanced by the corresponding ligand stimulation . Further in vitro protein block analysis confirmed the inclusion of a new retinoid receptor response element in the subclone. This element sequence is extremely conserved, consisting of a repeated sequence of 6 bases