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目的探讨地塞米松诱导的危重病性肌病的细胞死亡方式。方法将SD大鼠分为健康对照组和实验组;实验组又分为7、9、11 d 3个时相点(n=10)。实验组采用5 mg/kg地塞米松连续腹腔注射,每天1次,健康对照组腹腔注射等量的生理盐水。采用肌功能缺损评分判定肌功能缺损情况。利用免疫组化和Western blot检测比目鱼肌activecaspase-3、PARP、pULK的表达情况。结果①健康对照组无肌肉功能缺损症状;而实验组7 d时大鼠70%出现肌肉功能缺损症状,9、11 d时全部出现明显的肌肉功能缺损症状,以11 d时相点缺损程度最为严重。②各组active caspase-3、PARP均为阴性表达;健康对照组可见少量pULK阳性表达细胞,实验组pULK表达显著增强,以11 d时相点表达增强最为明显。结论地塞米松诱导的危重病性肌病中无active caspase-3、PARP表达,以pULK表达为主,提示该病可能以自噬性死亡为主要死亡方式。
Objective To investigate the cell death pattern of dexamethasone-induced critically ill myopathy. Methods The SD rats were divided into healthy control group and experimental group. The experimental group was divided into 3 groups at 7, 9 and 11 days (n = 10). The experimental group received 5 mg / kg dexamethasone continuous intraperitoneal injection once a day. The healthy control group was injected with the same amount of normal saline. Muscular function deficit score was used to determine muscle dysfunction. Immunohistochemistry and Western blot were used to detect the expression of active caspase-3, PARP and pULK in soleus muscle. Results ① There were no symptoms of muscle dysfunction in the healthy control group; 70% of the rats in the experimental group showed symptoms of muscle dysfunction on the 7th day, and all the muscle dysfunction symptoms appeared on the 9th and 11th days. At the 11th day, serious. ② The positive expression of active caspase-3 and PARP in each group was negative. A small amount of pULK positive cells were found in healthy control group. The expression of pULK in experimental group was significantly increased, especially in 11d. Conclusion No active caspase-3 and PARP are expressed in dexamethasone-induced critically ill myopathy. The expression of pULK is predominant, suggesting that autophagic death may be the predominant death pattern.