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以“陕林4号”和“07-69×青1”为试材,分别用浓度为0、5、10、15、20mmol/L的CaCl2溶液喷施杨树幼苗,4℃低温胁迫2d后测定杨树幼苗的膜伤害率、丙二醛(MDA)含量、抗氧化酶(SOD和POD)活性以及可溶性蛋白质含量等生理指标,以探究低温胁迫下不同浓度外源Ca2+对杨树幼苗抗寒相关生理指标的影响。结果表明:在喷施10mmol/L CaCl2的作用下,杨树幼苗的各项抗寒相关生理指标最优,可显著降低杨树幼苗的膜伤害率,减少丙二醛(MDA)含量,提高超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性,增加可溶性蛋白质含量。外源Ca2+可以有效提高杨树幼苗的抗寒能力,缓解低温胁迫对杨树幼苗的伤害。
Poplar seedlings were sprayed with CaCl2 solution of 0, 5, 10, 15 and 20 mmol / L, respectively, with “Shanyin 4” and “07-69 × Qing 1” After 2 days of low temperature stress, the membrane damage rate, malondialdehyde (MDA) content, antioxidant enzyme activities (SOD and POD) and soluble protein content of poplar seedlings were measured to investigate the effects of different concentrations of Ca2 + Effect of cold resistance related physiological indices of tree seedlings. The results showed that under the action of 10 mmol / L CaCl2, the cold-resistant physiological indexes of poplar seedlings were the best, which could significantly reduce the membrane damage rate, reduce the content of malondialdehyde (MDA) Oxidative dismutase (SOD) activity and peroxidase (POD) activity, increase the soluble protein content. Exogenous Ca2 + can effectively improve the cold resistance of poplar seedlings and alleviate the damage to poplar seedlings caused by low temperature stress.