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目的分析苯甲酸雌二醇(estradiol benzoate,EB)预处理的去势大鼠经红藻氨酸(kainic acid,KA)诱导性发作后海马基因表达的图谱,探讨雌激素对性发作大鼠海马的影响。方法应用含有10000个基因的cDNA芯片,检测EB干预对KA诱导的去势大鼠性发作后海马组织基因表达的影响。应用功能富集分析,筛选有统计学差异的基因功能群。结果EB逆转了KA致后有显著差异表达的基因共392个,其中下调的基因258个(65.82%),上调的基因134个(34.18%)。经功能富集分析,共筛选出8个主要功能群,其中下调的功能群5个(共21个基因),主要涉及凋亡、抗凋亡与神经发生、长时程突触传递增强效应等;上调的功能群有3个(共4个基因),涉及细胞膜受体相关的信号转导等。结论EB能逆转KA诱导的去势大鼠性发作后海马神经元的基因表达,且可能以促进神经元凋亡为主。
OBJECTIVE: To analyze the gene expression profile of hippocampus induced by kainic acid (KA) induced estradiol benzoate (EB) preconditioning in ovariectomized rats. Effects of rat hippocampus. Methods The cDNA microarray containing 10000 genes was used to detect the effects of EB on the gene expression of hippocampus after KA-induced ovariectomized rats. Application of functional enrichment analysis, screening of statistically significant differences in gene function groups. Results EB reversed a total of 392 genes that were significantly differentially expressed after KA toxin, of which 258 (65.82%) were down-regulated and 134 (34.18%) were up-regulated. After enrichment analysis, a total of 8 major functional groups were screened, of which 5 were functional groups (21 genes in total), mainly related to apoptosis, anti-apoptosis and neurogenesis, long-term potentiation of synaptic transmission and the like ; Up-regulated functional group has 3 (a total of 4 genes) involved in cell membrane receptor-related signal transduction. Conclusion EB can reverse the gene expression of hippocampal neurons after KA-induced castration in castrated rats, and may mainly promote neuronal apoptosis.