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目的建立一种稳定、灵敏的方法用于检测 IL - 4生物活性。方法比较人外周血 T淋巴细胞与 CTL L - 2 /IL - 4R细胞系对 rh IL - 4的增殖应答特点及 MTT法用于 rh IL - 4生物活性检测过程中的细胞浓度、细胞培养时间、MTT掺入浓度及时间等条件。结果两者均能对 rh IL - 4呈规律性增殖应答。 IL - 4活性测定时 ,人外周血 T淋巴细胞检测最低限为 8U /ml,CTL L - 2 /IL - 4R细胞系为 0 .73U /ml。确定了 MTT法用于 CTL L - 2 /IL - 4R细胞系检测 rh IL - 4生物活性时的最佳条件方案。结论就重复性和应答敏感性来说 ,CTL L - 2 /IL - 4R较之 T淋巴细胞更为优越 ,该细胞系可用于建立一种较灵敏而稳定的 IL -4测活方法。
Objective To establish a stable and sensitive method for the detection of IL - 4 biological activity. Methods The proliferative responses of human peripheral blood T lymphocytes and CTL L - 2 / IL - 4R cell lines to rhIL - 4 were compared. MTT assay was used to detect the concentration of rhIL - 4, the cell culture time, MTT incorporation concentration and time and other conditions. Results Both showed a regular proliferative response to rhIL - 4. When IL - 4 activity was measured, the detection limit of T lymphocytes in human peripheral blood was 8U / ml, while the CTL L - 2 / IL - 4R cell line was 0.73U / ml. MTT assay was used to determine the optimal conditions for detecting the biological activity of rhIL - 4 in CTL L - 2 / IL - 4R cell line. Conclusion CTL L - 2 / IL - 4R is superior to T lymphocytes in terms of reproducibility and response sensitivity. This cell line can be used to establish a more sensitive and stable IL - 4 assay.