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目的:建立柴银口服液中连翘苷的HPLC的含量测定方法,测定5个不同批号柴银口服液中连翘苷的含量,为更好的控制柴银口服液的质量提供参考。方法:过中性氧化铝获得供试品溶液,HPLC测定其含量;色谱条件为十八烷基硅烷键合硅胶色谱柱;流动相乙腈-水(30∶70);流速1 mL.min-1;柱温室温;检测波长277 nm。结果:采用此HPLC法检测柴银口服液在0.82～2.87μg(r=0.999 9),表明线性关系良好,回归方程为Y=620.01X+41.092;回收率为101.78%,RSD为0.72%。结论:该方法能够为柴银口服液的质量控制提供参考。
OBJECTIVE: To establish a method for the determination of forsythin in Chaiyin Oral Liquid and to determine the content of forsythin in five different batches of Chai-Yin oral liquid for reference to better control the quality of Chaiyin Oral Liquid. Methods: The sample solution was obtained by neutral alumina. The content was determined by HPLC. The chromatographic conditions were octadecylsilane bonded silica gel column. The mobile phase was acetonitrile-water (30:70), the flow rate was 1 mL.min-1 ; Column temperature at room temperature; detection wavelength 277 nm. Results: The HPLC method was used to detect Chaiying oral liquid at 0.82-2.87μg (r = 0.999 9), which showed a good linearity. The regression equation was Y = 620.01X + 41.092. The recovery rate was 101.78% and the RSD was 0.72%. Conclusion: This method can provide a reference for the quality control of Chai Yin Oral Liquid.