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目的:考察栀子水提液中栀子苷、西红花苷I的稳定性并优选栀子的减压提取工艺参数。方法:采用经典恒温法考察栀子水提液中栀子苷、西红花苷I的稳定性。以栀子苷、西红花苷I含量及浸膏得率为因变量,提取温度、提取时间、料液比为自变量,利用Box-Behnken设计-效应面法优选提取工艺条件。结果:最佳提取工艺参数为提取温度45℃,提取时间60 min,料液比1∶14;栀子苷质量分数3.51%,西红花苷I质量分数1.21%,浸膏得率28.24%,RSD分别为1.44%,2.21%,0.96%,各因变量与预测值偏差较小。结论:减压提取法适合药材中热敏性成分的提取,优选的减压工艺稳定可行,为栀子的综合利用开发提供实验依据。
OBJECTIVE: To investigate the stability of geniposide and crocin I in the aqueous extract of Gardenia jasminoides and optimize the extraction parameters of gardenia under reduced pressure. Methods: The stability of geniposide and crocin I in Fructus Gardeniae was studied by classical thermostat method. Taking the concentration of geniposide, crocin I and the yield of extract as the dependent variable, the extraction temperature, the extraction time and the ratio of solid to liquid as the independent variables, the optimum extraction conditions were obtained by the Box-Behnken design-response surface method. Results: The optimal extraction parameters were extraction temperature 45 ℃, extraction time 60 min, solid-liquid ratio 1:14, mass fraction of gardenoside 3.51%, caryol I mass fraction 1.21% and extraction rate 28.24% RSD were 1.44%, 2.21% and 0.96% respectively, and the deviation of each dependent variable from the predicted value was small. Conclusion: The decompression extraction method is suitable for the extraction of thermosensitive components in medicinal materials. The optimal decompression process is stable and feasible, which provides the experimental basis for the comprehensive utilization of Gardenia jasminoides.