硫酸软骨素是从生物体内提取出来的一种天然药物 .本文采用反相高效液相色谱法 ,利用外标法定量 ,测定了硫酸软骨素的含量 .色谱柱为ZORBAX—ODS( 4 .6mm× 150mm ) ,流动相为水∶甲醇∶0 .0 1mol/磷酸盐缓冲液 ( pH4 ) =4 70∶2 0∶10 ,检测波长 2 0 0nm ,平均回收率为 98.2 6% ,RSD为1.0 5% (n =5) .本法具有快速、简便、灵敏、准确等优点 Chondroitin sulfate is a natural drug extracted from the body. In this paper, RP-HPLC was used to determine the content of chondroitin sulfate by external standard method. The column was ZORBAX-ODS ( 4 .6mm × 150mm ), the mobile phase was water : methanol : 0. 0 1mol/phosphate buffer (pH4) = 4 70:2 0:10, the detection wavelength was 200nm, the average recovery was 98.26%, RSD was 1.05% (n = 5) . This method is fast, simple, sensitive and accurate