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目的探讨小鼠感染疟疾后是否会对艾滋病疫苗的免疫产生影响。方法用HIV-1 DNA疫苗p VAX-gag分别免疫感染过疟疾和未感染过疟疾的Balb/c小鼠,观察不同组小鼠接种p VAX-gag疫苗后,细胞免疫、体液免疫和免疫记忆的变化。结果p VAX1-gag免疫感染过疟疾和未感染过疟疾的Balb/c小鼠,均可诱导出明显的体液免疫和细胞免疫反应;但p VAX1-gag免疫未感染过疟疾的小鼠诱导出的体液免疫反应高于感染过疟疾的小鼠,所获得的抗体滴度是感染过疟疾的小鼠的3倍,未感染过疟疾的小鼠诱导出的细胞免疫反应高于感染过疟疾的小鼠。结论感染疟疾后对艾滋病疫苗的免疫有一定的抑制作用,这为艾滋病疫苗的研发提供了实验依据。
Objective To investigate whether the mice infected with malaria will have an impact on the immunization of AIDS vaccine. Methods Balb / c mice infected with malaria and non-infected malaria were immunized with HIV-1 DNA vaccine p VAX-gag respectively. The cellular immunity, humoral immunity and immune memory of different groups of mice vaccinated with p VAX-gag Variety. RESULTS: Significant humoral and cellular immune responses were induced in both Balb / c mice immunized with p VAX1-gag and malaria-naïve malaria; however, mice immunized with p VAX1-gag did not induce malaria The humoral immune response was higher in mice infected with malaria than in mice infected with malaria. The antibody titers obtained were three times those of mice infected with malaria. Mice that had not been infected with malaria induced higher cellular immune responses than those infected with malaria . Conclusion The malaria infection has a certain inhibitory effect on the immunization of AIDS vaccine, which provides experimental evidence for the development of AIDS vaccine.