能否有效地控制分泌特异性单克隆抗体 (Mc Ab)杂交瘤细胞的污染 ,是该项研究中常遇到的问题 ,由于细菌耐药性的产生 ,传统应用双抗处理的方法多难于奏效。在杂交瘤细胞融合培养过程中 ,有时会发生真菌污染的现象。为防止和清除真菌污染 ,除了严格控制操作 ,还可采用小鼠腹腔和 The ability to effectively control the contamination of secreted monoclonal antibody (McAb) hybridomas is a common problem in this study. Traditionally, the use of double-antibody-based methods has proved more difficult due to bacterial resistance. In hybridoma cell culture, fungal contamination sometimes occurs. In order to prevent and eliminate fungal contamination, in addition to strict control of the operation, mice can also be used intraperitoneally