论文部分内容阅读
[目的]观察低功率密度微波辐射对小鼠学习记忆能力的影响。[方法]将40只雄性昆明小鼠随机分成对照组及低、中、高3个微波辐射暴露组(500、1 000、2 000μW/cm~2),每组10只,每天照射1 h,连续照射30 d。照射30 d后进行Y迷宫训练连续7 d,观察小鼠学习记忆能力变化;取海马组织匀浆,用高效液相色谱法观察海马组织中氨基酸含量的变化。[结果]观察学习能力的Y迷宫实验中,与对照组(54.62±3.13)s相比,3个暴露组小鼠的逃避潜伏期[分别(76.24±4.17)s、(95.36±2.31)s、(117.01±4.13)s]均有所延长(P<0.05);中、高暴露组小鼠进入错误区域的次数[(7.24±0.57)、(9.86±0.28)次/min]也较对照组[(2.67±0.38)次/min]增加(P<0.05);微波辐射后海马组织中谷氨酸的质量分数减少,γ-氨基丁酸增加(P<0.05)。[结论]低功率密度微波辐射对小鼠的学习记忆能力有损害,可能是通过γ-氨基丁酸的增加和谷氨酸减少实现的。
[Objective] To observe the effect of low power density microwave radiation on learning and memory in mice. [Methods] Forty male Kunming mice were randomly divided into control group and low, middle and high exposure groups (500, 1 000, 2 000 μW / cm 2) Continuous irradiation for 30 d. Y maze training was performed 30 days after irradiation for 7 consecutive days to observe the change of learning and memory ability of mice. Hippocampus tissue homogenate was used to observe the changes of amino acid content in hippocampus tissue by high performance liquid chromatography. [Results] Compared with the control group (54.62 ± 3.13) s, the escape latency of the three exposed mice [(76.24 ± 4.17) s, (95.36 ± 2.31) s, 117.01 ± 4.13) s] (P <0.05). The number of mice in middle and high exposure groups was also higher than that in control group [(7.24 ± 0.57), (9.86 ± 0.28) / min) 2.67 ± 0.38) times / min] (P <0.05). The content of glutamate in hippocampus decreased and the content of γ-aminobutyric acid increased after microwave irradiation (P <0.05). [Conclusion] Low power density microwave radiation can impair the ability of learning and memory in mice, possibly through the increase of γ-aminobutyric acid and the reduction of glutamate.