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目的 观察弓形虫表面抗原 P30 DNA疫苗免疫 BAL B/ c小鼠诱导其体内产生的体液免疫应答及抗虫感染的免疫保护作用。 方法 重组质粒 p BK- P30用生理盐水稀释后 ,肌注免疫 BAL B/ c小鼠。分别于免疫后 5周和 10周 ,EL ISA测定 Ig G抗体滴度 ;取免疫鼠的血液、肺、心脏、肝脏、脾、肾脏及肌肉 PCR扩增 P30基因 ;免疫鼠腹腔注射弓形虫速殖子攻击感染。 结果 两次检测 ,均可测到特异性 Ig G抗体 ,且抗体的滴度随免疫时间的延长而增高 ;免疫后 5周 ,免疫鼠的上述组织均可扩增出 P30基因条带 ,但免疫后 10周仅血液扩增出特异 P30基因条带 ;弓形虫速殖子腹腔攻击感染 ,免疫组鼠的平均存活时间较对照组鼠延长 ,但统计学差异不显著 (P>0 .0 5 )。 结论 弓形虫表面抗原 P30DNA疫苗能诱导 BAL B/ c小鼠产生特异性体液免疫应答及部分抗虫免疫保护作用。
Objective To observe the humoral immune response induced by P30 DNA vaccine of Toxoplasma gondii in BALB / c mice and the immunoprotective effect of insect-resistant infection. Methods BALB / c mice were immunized intraperitoneally with recombinant plasmid pBK-P30 diluted with saline. IgA antibody titers were measured by EL ISA at 5 weeks and 10 weeks after immunization. The P30 gene was amplified by PCR from the blood, lung, heart, liver, spleen, kidney and muscle of immunized mice. The immunized mice were injected intraperitoneally with Toxoplasma gondii Sub-attack infection. Results The results of two tests showed that the specific Ig G antibody was detected, and the titer of the antibody increased with the increase of the immunization time. At 5 weeks after immunization, the above tissues of the immunized mice could amplify the P30 gene band but the immunization Only the P30 gene band was amplified in the blood only 10 weeks later. Toxoplasma gondii attacked by intraperitoneal challenge. The average survival time in the immunized group was longer than that in the control group, but the difference was not statistically significant (P> 0.05) . Conclusion The Toxoplasma gondii surface antigen P30 DNA vaccine can induce specific humoral immune response and some insect-resistant immunoprotective effects in BALB / c mice.