已知多糖与塑料和聚苯乙烯的亲和力极低,在RIA 或EIA 试验中难以包被,如用多聚赖氨酸、生物素和酪胺修饰也难以取得良好的重现性,且空白值高,多糖易丧失特异性和抗原性。作者等应用真空抽吸法可将天然的、不需修饰的多糖固定于不同孔径和型号的硝酸纤维素膜上,可用酶联免疫转印法筛查单个杂交瘤细胞培养液中的抗多糖抗体,也 It is known that polysaccharides have very low affinity for plastics and polystyrene, are difficult to coat in RIA or EIA tests, and are difficult to achieve good reproducibility with polylysine, biotin and tyramine modification, and blank values High, polysaccharide susceptibility to loss of specificity and antigenicity. Authors such as the application of vacuum suction method can be natural, without modification of polysaccharides fixed in different pore size and type of nitrocellulose membrane, ELISA screening of anti-polysaccharide antibodies in hybridoma cell culture medium ,and also