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目的探讨神经元型一氧化氮合酶（ｎＮＯＳ）在暂时性局灶性脑缺血中的作用。方法用栓线法建立了大脑中动脉阻塞（ＭＣＡＯ）模型的大鼠上，观察特异性ｎＮＯＳ抑制剂７－ｎｉｔｒｏ－ｉｎｄａｚｏｌｅ（７－ＮＩ）对大鼠缺血３ｈ、再灌注３ｈ脑梗塞灶范围的影响。结果７－ＮＩ（２５ｍｇ／ｋｇ）可减小大鼠脑梗塞灶范围，且主要减小大脑皮质梗塞灶，其作用可被Ｌ－精氨酸（３００ｍｇ／ｋｇ）逆转，Ｄ－精氨酸（３００ｍｇ／ｋｇ）则否。结论ｎＮＯＳ产生的ＮＯ在暂时性局灶性脑缺血中起损害作用
Objective To investigate the role of neuronal nitric oxide synthase (nNOS) in transient focal cerebral ischemia. Methods A rat model of middle cerebral artery occlusion (MCAO) was established by suture method. The effect of 7-nitro-indazole (7-NI), a specific nNOS inhibitor, on the infarct size Impact. Results 7-NI (25 mg / kg) reduced the extent of cerebral infarction in rats and mainly reduced the cerebral cortex infarction. The effect was reversed by L-arginine (300 mg / kg) 300mg / kg) is not. Conclusions NO produced by nNOS may play a detrimental role in transient focal cerebral ischemia