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微量热法被用于分析连续种植桉树Eucalyptus granddis×E.uophylla、甘蔗的地块的土壤微生物代谢活性。于雨季和旱季分别从接壤的种植桉树、甘蔗地块采集土壤样品(样地大小10m×10m,各采500g),加入含5.0mg葡萄糖和5.0mg硫酸铵的0.6mL溶液的1.2g土壤样品被用于微量热法实验,温度设定为28℃;另外,土壤的理化和生物学特性也被测定,用于共同反映土地经营模式对土壤质量的影响。结果:(1)无论是雨季还是旱季,甘蔗连续种植地土壤内发酵型细菌为土壤微生物主要类群,但桉树林地只有雨季时发酵型细菌占优势;(2)与甘蔗连续种植地相比,桉树林地的土壤密实度较低,土壤微生物组成和代谢活动的季节性波动程度较高;(3)相对于桉树,甘蔗的连续种植对土壤微生物代谢活性产生较大的抑制作用。
Microthermometry was used to analyze soil microbial metabolic activity of continuously planted Eucalyptus grandis × E.uophylla, plots of sugarcane. Soil samples (10 m × 10 m, 500 g each) were collected from bordering eucalyptus and sugarcane plots in rainy and dry seasons, respectively, and 1.2 g of soil samples containing 0.6 mL of a solution containing 5.0 mg of glucose and 5.0 mg of ammonium sulfate were added For the microcalorimetric experiment, the temperature was set at 28 ° C. In addition, the physicochemical and biological properties of soils were also measured to jointly reflect the impact of land management practices on soil quality. Results: (1) In the rainy and dry seasons, the fermentative bacteria in continuous cultivated soils of sugarcane were the main groups of soil microorganisms, but the fermentative bacteria were dominant in eucalyptus forest only in rainy season. (2) Compared with continuous planting of sugarcane, eucalyptus The soil density of woodland was lower, and the seasonal fluctuations of soil microbial composition and metabolic activities were higher. (3) Compared with eucalyptus, the continuous planting of sugarcane had a greater inhibitory effect on soil microbial metabolic activity.