当前食用菌的社会需求量越来越大,食用菌的大量生产增加了对菌种的需求。由于菌种的培养条件要求严格,特别是黑木耳的常规组织分离法,生产难度很大。经我们几年实验,总结出一种简便的组织分离方法,效果很好,现介绍如下: 把长满菌丝的优质木耳菌种瓶,放于树荫的花花阳光下,在22—24℃条件下光照诱耳。几天后,瓶内菌丝体便长出小米粒大小的棕黑色耳基来。按无菌操作,在接种箱内打碎菌种瓶,迅速用接种针挑取一点耳基及基底菌丝,接种于琼脂马铃薯培养基斜面上,在27℃条件下培养10天左右即可得到理想的“母种”。此法经与子实体制取母种法比较,成活率提高10多倍,为扩大再生提供了充足的种源。经实 The current social needs of mushrooms growing, mass production of mushrooms increased demand for bacteria. Due to the stringent culture conditions of bacteria, especially the black fungus, the conventional tissue separation method, the production of a great difficulty. After several years of our experiment, summed up a simple method of tissue separation, the effect is very good, are described as follows: The high quality mushroom fungus mycelium, placed in the shade of flowers and flowers, at 22-24 ℃ Light conditions induced lure. A few days later, the mycelium in the bottle grew brownish ear base of millet grain size. According to aseptic operation, inoculation box to break the bacteria bottle, pick the needle with a needle and quickly pick up the substrate mycelium, inoculated on agar potato culture slant surface, cultured at 27 ℃ for about 10 days can be obtained Ideal “mother species.” Compared with the mother plant method, the survival rate increased more than 10 folds, which provided a sufficient provenance for expanding regeneration. The real