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用 Yamamoto 报道的柱酶联免疫测定法,对130例各类肝病患者(其中急性肝炎20例,暴发性肝炎10例,慢性肝炎20例,肝硬化30例,肝细胞癌20例,肝内郁胆10例,肝外郁胆10例,酒精性肝病10例)及随机选择的30个健康供血员作为对照组进行分泌性免疫球蛋白 A(slgA)检测。同时用自动分析仪检测血清GOT、GPT、ALP、亮氨酸氨肽酶(LAP)、总胆红素(TBil)和直接胆红素及用 Tri-partigen-IgA 板的单向辐射免疫测定血清总 IgA 以作比较。
Using Yamamoto column ELISA, 130 cases of various types of liver disease patients (including 20 cases of acute hepatitis, fulminant hepatitis in 10 cases, 20 cases of chronic hepatitis, 30 cases of cirrhosis, hepatocellular carcinoma in 20 cases, 10 cases of gallbladder, 10 cases of extrahepatic cholestasis, 10 cases of alcoholic liver disease) and 30 healthy blood donors randomly selected as the control group for the detection of secreted immunoglobulin A (slgA). Serum GOT, GPT, ALP, leucine aminopeptidase (LAP), total bilirubin (TBil), direct bilirubin and immunoassay of serum with Tri-partigen-IgA plate were detected by automatic analyzer Total IgA for comparison.