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目的原核表达并纯化SARS冠状病毒N蛋白。方法用RT-PCR技术克隆SARS冠状病毒PUMC2株N蛋白全长cDNA,cDNA经序列分析鉴定后,克隆到pET32a表达载体,转化E.coliBL21进行原核表达并纯化出SARS冠状病毒N蛋白。结果实现了SARS冠状病毒N蛋白的表达及纯化。结论用基因重组方法表达的SARS冠状病毒N蛋白可为其进一步功能研究提供条件。
Objective To prokaryotic express and purify SARS-CoV N protein. Methods The full-length cDNA of N protein of SARS coronavirus PUMC2 was cloned by RT-PCR. The cDNA was identified by sequence analysis, cloned into pET32a expression vector, transformed into E.coli BL21 for prokaryotic expression and purified SARS-CoV N protein. The results achieved the SARS coronavirus N protein expression and purification. Conclusion The SARS-CoV N protein expressed by gene recombination method may provide conditions for its further function research.