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目的:系统进行戊二醛固定的异种血管的免疫学研究及其动物在体排斥反应强度。方法:实验分两组:新鲜未处理组和戊二醛固定组。每一组分术后第4天、12天、30天和60天四个时间点。分别通过对20例新鲜猪肺动脉、20例戊二醛固定的异种血管进行大鼠皮下包埋,在术后第4天、12天、30天和60天进行观察,利用病理学和免疫组织化学等技术,全面系统比较了两组病理学、巨噬细胞CD68、补体C3、先天性抗体Ig G形态性变化及其IOD值变化。结果:HE染色结果显示新鲜组在术后第12天、30天染色较强,戊二醛组以上指标术后第12天、30天染色比新鲜组较弱。CD68、C3、Ig G值变化结果相似;免疫组织化学累积光密度(Integral optical density,IOD)定量检测结果显示:IOD值术后第4天开始升高,到第12天、30天达到峰值,第60天维持或下降。结论:先天性免疫在探索异种免疫排斥机理有重要作用;戊二醛固定的异种血管免疫原性下降,但是仍然具有免疫原性,需要探索更好的方法来削弱排斥反应。
OBJECTIVE: To systematically conduct immunological studies of glutaraldehyde-fixed xenogeneic vessels and their in vivo rejection response. Methods: The experiment was divided into two groups: fresh untreated group and glutaraldehyde fixed group. Four points, four days, 12 days, 30 days and 60 days after each component were observed. Rats were subcutaneously embedded in 20 fresh porcine pulmonary arteries and 20 glutaraldehyde-fixed xenografts, respectively. The rats were observed on the 4th, 12th, 30th and 60th days after operation. Pathological and immunohistochemical And other techniques, a comprehensive and systematic comparison of the two pathology, macrophages CD68, complement C3, congenital antibodies Ig G morphological changes and IOD values. Results: HE staining showed that the staining of fresh group was stronger on the 12th day and the 30th day after operation. The above indexes of the glutaraldehyde group were weaker on the 12th day and the 30th day after the operation than the fresh group. The values of CD68, C3 and IgG showed similar changes. The results of immunohistochemistry (IOD) showed that the IOD increased from the 4th day after operation to the peak on the 12th and 30th day, The first 60 days to maintain or decline. CONCLUSION: Congenital immunity plays an important role in exploring the mechanism of xenograft rejection. The immunogenicity of glutaraldehyde-fixed xenogeneic vessels is decreased, but still remains immunogenic. There is a need to explore better ways to attenuate rejection.