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The investigation of fingerprint of Yubaizhi was performed by high-performance liquid chromatography (HPLC). Chromatographic conditions were: the mobile phase was the mixture of acetonitrile-water (43:57, v/v); the flow rate was 1mL/min; the column temperature was 40℃; the detection wavelength was 254nm. The stability, precision and reproducibility were performed according to "Specification of Fingerprint of Injection of Chinese Traditional Medicine (temporarily)" regulated by State Food and Drug Administration(SFDA). The HPLC fingerprints of Yubaizhi from various locations were well constant.