应用CM—Sephadex C—50离子交换柱层析分离福建眼镜王蛇毒,获得17个蛋白峰。该蛇毒具有磷脂酶A_2、蛋白水解酶、精氨酸酯酶、L-氨基酸氧化酶、胆碱酯酶、磷酸单酯酶和磷酸二酯酶等酶活力。组分Ⅰ,Ⅳ对ADP诱导的兔血小板聚集功能有抑制作用,组分Ⅰ还有降压作用,组分Ⅳ则有心脏毒性作用。Ⅵ,Ⅶ,Ⅷ,Ⅸ,Ⅹ,Ⅺ和Ⅻ等7个组分具突触后神经毒活性,组分ⅩⅫ呈现局部出血效应,粗毒及各组分对家兔血浆的复钙时间和凝血酶时间无影响。 Fujian King Cobra venom was isolated by CM-Sephadex C-50 ion exchange chromatography and 17 protein peaks were obtained. The snake venom has phospholipase A_2, proteolytic enzymes, arginine esterase, L-amino acid oxidase, cholinesterase, phosphomonoesterase and phosphodiesterase and other enzyme activity. Components Ⅰ, Ⅳ inhibit ADP-induced rabbit platelet aggregation function, component Ⅰ still has antihypertensive effect, while component Ⅳ has cardiotoxic effect. Seven components of Ⅵ, Ⅶ, Ⅷ, Ⅸ, Ⅹ, Ⅺ and Ⅻ had post-synaptic neurotoxicity, while component ⅩⅫ showed local hemorrhage. The effects of crude toxicity and each component on rabbit plasma calcium reabsorption time and coagulation No effect of enzyme time.