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目的建立一种新型大鼠脊髓切割损伤模型,为临床脊髓不同平面损伤研究提供适宜的动物模型。方法45只SD大鼠随机分为脊髓不同平面双侧半横切组(A组,n=15)、脊髓全横切组(B组,n=15)和假手术组(C组,n=15)。术后观察、记录各组大鼠活动、进食水、排尿排便、切口感染等并发症和死亡情况,采用BBB运动功能评分和斜板试验评价大鼠行为学功能。结果术后A组大鼠活动、进食水量减少,53.33%大鼠出现尿潴留,40.00%大鼠于术后1~4周死亡;B组大鼠活动、进食水量明显减少,66.67%大鼠出现尿潴留,60.00%大鼠于术后1~4周死亡;C组大鼠活动、进食水量无明显变化,无并发症及死亡发生。BBB运动功能评分及斜板维持最大角度值检测显示,A、B组均低于C组(P<0.05),而这些评价指标在A组和B组之间无显著差异。结论脊髓不同平面双侧半横切损伤模型大鼠尿潴留及死亡率低,行为学评价与脊髓全横切损伤模型类似,适于脊髓不同平面双侧损伤修复的研究。
Objective To establish a new model of spinal cord injury in rats and to provide a suitable animal model for the study of different planar injuries in clinical spinal cord. Methods Forty-five Sprague-Dawley rats were randomly divided into two groups (A group, n = 15), spinal cord transection group (B group, n = 15) and sham operation group 15). After operation, the complication and death of rats in each group were recorded, such as activity of the rats, water intake, defecation of the urine, incision infection and so on. The BBB motor function score and the inclined plate test were used to evaluate the behavioral function. Results After operation, the activity of rats in group A decreased, the water intake decreased, 53.33% rats had urinary retention, and 40.00% rats died 1 ~ 4 weeks after operation. The activity of rats in group B decreased significantly, and the amount of water consumed decreased significantly, 66.67% Urinary retention, 60.00% rats died 1 to 4 weeks after operation; rats in group C had no significant changes in water intake, no complication and death. The score of BBB motor function and the maximum angle of slope maintenance showed that the values of A, B were lower than those of C (P <0.05), but there was no significant difference in these indexes between A and B groups. Conclusions The rats with bilateral semi-transverse transection injury in the spinal cord have lower urinary retention and mortality. The behavioral evaluation is similar to that of the spinal cord transection injury model, and is suitable for the study of bilateral planar spinal cord injury repair.