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目的观察肝素对大鼠心肺复苏后24h神经细胞Bcl-2、Bax表达的影响及保护作用。方法 30只雄性SD大鼠随机均分为单纯肾上腺素组(A组)、肝素组(H组)和假手术组(C组)。麻醉后行气管插管和动静脉置管,A组和H组建立窒息性心跳骤停模型。窒息8min后开始心肺复苏:A组给予肾上腺素0.01mg/kg+生理盐水,H组给予肾上腺素0.01mg/kg+肝素0.5mg/kg,C组不窒息。麻醉前、复苏后24h行神经功能损害(ND)评分,免疫组化方法检测复苏后24h大鼠大脑皮质和海马神经元Bcl-2、Bax的表达。结果与A组相比,H组ND评分降低(P<0.05);与C组相比,A组和H组Bcl-2和Bax表达均增加,H组Bcl-2增加更显著,A组Bax增加更显著(P<0.01)。结论肝素可通过增加Bcl-2表达、减少Bax表达而减轻脑损伤。
Objective To observe the effect of heparin on the expression of Bcl-2 and Bax in neurons after cardiopulmonary resuscitation (24h) in rats and its protective effect. Methods Thirty male Sprague-Dawley rats were randomly divided into simple epinephrine group (A group), heparin group (H group) and sham operation group (C group). After anesthesia tracheal intubation and arteriovenous catheterization, A group and H group to establish asphyxial cardiac arrest model. Cardiac resuscitation was started 8 minutes after asphyxiation: group A was treated with 0.01 mg / kg epinephrine and saline, while group H was given 0.01 mg / kg epinephrine and 0.5 mg / kg heparin. Group C was not asphyxiated. Nerve function impairment (ND) was assessed 24h before and 24 h after resuscitation. The expression of Bcl-2 and Bax in cerebral cortex and hippocampus neurons was detected by immunohistochemistry. Results Compared with group A, the ND score of group H decreased (P <0.05). Compared with group C, the expression of Bcl-2 and Bax in group A and group H increased, while in group B, Bcl-2 increased more significantly Increase more significantly (P <0.01). Conclusion Heparin reduces brain injury by increasing Bcl-2 expression and decreasing Bax expression.