论文部分内容阅读
目的探讨氯丙咪嗪对人脑胶质瘤U251细胞体外增殖的影响。方法用甲基噻唑基四唑(MTT)比色法检测不同浓度的氯丙咪嗪对U251细胞增殖活性的影响。根据结果,选取二个不同浓度组检测细胞形态学的变化、台盼兰染色细胞死亡计数、细胞凋亡染色、流式细胞术检测等。结果依据MTT实验结果,选取氯丙咪嗪57μmol/L和28.5μmol/L两个浓度进行台盼兰染色、细胞形态学、细胞凋亡染色和流式细胞术检验。结果证实,氯丙咪嗪对U251细胞有明显的抑制作用。结论氯丙咪嗪具有明显的抑制胶质瘤U251细胞增殖的功能,促进其凋亡,可作为胶质瘤化疗的备选药物。
Objective To investigate the effect of clomipramine on the proliferation of human glioma U251 cells in vitro. Methods Methyl thiazolyl tetrazolium (MTT) colorimetry was used to detect the effect of different concentrations of clomipramine on the proliferation of U251 cells. According to the results, two different concentration groups were selected to detect cell morphological changes, trypan blue staining of cell death count, apoptosis staining, flow cytometry and so on. Results According to MTT results, two concentrations of clomipramine 57μmol / L and 28.5μmol / L were selected for trypan blue staining, cell morphology, apoptosis staining and flow cytometry. The results confirmed that clomipramine U251 cells have a significant inhibitory effect. Conclusion Clomipramine can significantly inhibit the proliferation of glioma U251 cells and promote the apoptosis of glioma U251 cells. It may be used as an alternative drug for glioma chemotherapy.