本文报告,用EB病毒转化的人体类B淋巴母细胞GM3299和GM4408在39～42℃培养时能合成γ干扰素(IFN-γ).GM3299在37℃培养时能自发产生IFN,水平约为1,000U/10~6细胞,若移至42℃培养时,60分钟内IFN活性增高,90～240分钟时达高峰,活性为20,000U/10~6细胞,而360分钟时活性低于原来水平.这可能由IFN自限性调节作用引起. The present report reports that human EBV-transformed human B lymphoblasts, GM3299 and GM4408, synthesize IFN-γ when cultured at 39 to 42 ° C. GM3299 spontaneously produces IFN at 37 ° C at a level of approximately 1,000 When cultured at 42 ℃, the activity of IFN increased within 60 minutes and peaked at 90 to 240 minutes. The activity was 20,000 U / 10 ~ 6 cells, while the activity was lower than the original level at 360 minutes. This may be caused by IFN self-regulation.