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目的:探讨褪黑素(MT)对常压下慢性间歇缺氧损伤SD大鼠心肺的保护作用。方法:将24只雄性,5周SD大鼠随机分成三组并模拟人类睡眠时间:对照组在常压常氧下饲养加灌服生理盐水,实验组分为实验1组常压低氧箱内饲养加灌服褪黑素、实验2组在常压低氧箱内饲养同时加灌服生理盐水,8周后检测体重(BW)、右心室/左心室加室间隔比值RV/(LV+S),右心室/体质量(RV/BW)比值以及心肌和肺组织谷胱苷肽过氧化物酶(GSH-PX)活力、超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)的变化。结果:实验2组与实验1组比较大BW明显降低(P<0.05),而RV/(LV+S)及RV/BW明显增加(P<0.05),心肌及肺组织GSH-PX活力、SOD活性、T-AOC均明显降低(P<0.05);实验1组与对照组比较BW无明显差异(P>0.05),而RV/(LV+S)及RV/BW无明显差异(P>0.05),心肌及肺组织GSH-PX活力、SOD活性、T-AOC均无明显差异(P>0.05);实验2组与对照组比较BW明显降低(P<0.05),而RV/(LV+S)及RV/BW却增加(P<0.05),心肌及肺组织GSHPX活力、SOD活性、T-AOC均明显差异(P<0.05)。结论:褪黑素可防止慢性间歇缺氧对心肺的损伤作用。
Objective: To investigate the protective effect of melatonin (MT) on cardiopulmonary of SD rats induced by chronic intermittent hypoxia. Methods: Twenty-four male and five-week-old SD rats were randomly divided into three groups and simulated human sleep time. The control group was fed with normal saline under normal pressure and normoxia. The experimental group was divided into experimental group 1 Feeding plus melatonin, the experimental group 2 were fed with normal saline in the hypobaric chamber at the same time, after 8 weeks, the body weight (BW), RV / LV + S (RV / BW) and the activities of glutathione peroxidase (GSH-PX), superoxide dismutase (SOD) and total antioxidant capacity in myocardial and lung tissue (T- AOC) changes. Results: Compared with experimental group 1, the BW in experimental group 2 was significantly decreased (P <0.05), and RV / (LV + S) and RV / BW were significantly increased (P <0.05). There was no significant difference in BW between the experimental group 1 and the control group (P> 0.05), but there was no significant difference between the RV / (LV + S) (P> 0.05). The BW and GSH-PX activity, SOD activity and T-AOC in myocardium and lung tissue were not significantly different between experimental group 2 and control group (P <0.05) ) And RV / BW increased (P <0.05). GSHPX activity, SOD activity and T-AOC in myocardium and lung tissue were significantly different (P <0.05). Conclusion: Melatonin can prevent chronic intermittent hypoxia injury of heart and lung.