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目的研究杨梅素(myricetin,Myr)对人肺腺癌(A549)细胞增殖的影响及其作用机制。方法以人肺腺癌系A549细胞为离体研究对象,通过噻唑蓝(MTT)法研究不同浓度的Myr对A549细胞生长的抑制作用并测定其半数抑制浓度(IC50);采用Westernblot法检测Myr对蛋白激酶B(Akt)磷酸化水平的影响。结果Myr对A549细胞具有明显的增殖抑制作用,随着Myr浓度的增加,细胞的存活率明显降低。Myr作用72h的IC50值为41.7μg/ml。32μg/ml杨梅素无血清作用A54912h即可显著抑制AktSer473的磷酸化水平。结论Myr能够剂量依赖性抑制A549细胞增殖,Akt的活性下调可能是Myr体外诱导人A549肺腺癌细胞增殖抑制作用的分子靶点。
Objective To study the effect of myricetin (Myr) on the proliferation of human lung adenocarcinoma (A549) cells and its mechanism. Methods Human lung adenocarcinoma A549 cells were isolated from human lung adenocarcinoma cell line A549. MTT assay was used to study the inhibitory effect of different concentrations of Myr on the growth of A549 cells. The half inhibitory concentration (IC50) was determined by Western blot. Effect of protein kinase B (Akt) phosphorylation. Results Myr had a significant inhibitory effect on A549 cells. With the increase of Myr concentration, the cell survival rate was significantly decreased. The IC50 of Myr for 72h was 41.7μg / ml. The myelin at 32μg / ml could significantly inhibit the phosphorylation of AktSer473 at A54912h. Conclusion Myr can inhibit the proliferation of A549 cells in a dose-dependent manner. The down-regulation of Akt may be the molecular target of Myr inducing the proliferation inhibition of human lung adenocarcinoma A549 cells in vitro.