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IL - 8作为一种趋化细胞因子在炎症反应中具有重要作用 ,其在内皮细胞内的表达受多种细胞因子的调节。本文用 TNF-α孵育培养的人脐静脉内皮细胞不同时间后 ,用 RT- PCR法检测内皮细胞内 IL - 8m RNA的表达 ,并用免疫细胞化学染色法检测内皮细胞内 NF-κB的激活。结果发现 :(1)未用 TNF-α孵育的内皮细胞 IL - 8表达量很少 ,TNF-α孵育 1小时后 IL - 8表达明显增加 ,3 h进一步增加 ;6 h IL - 8表达降低 ,9h进一步降低至基础水平 ;(2 )未用 TND-α孵育的内皮细胞核 NF-κB p6 5免疫细胞化学染色呈阴性 ,NF-α孵育 1,3 h后 NF-κB p6 5免疫细胞化学染色呈阳性 ,6 h时后呈弱阳性 ,9h后呈阴性。提示 :TNF-α可诱导内皮细胞表达 IL - 8并可激活内皮细胞内 NF-κB,二者的时相过程基本一致。作者认为 TNF-α诱导 IL - 8在内皮细胞内表达可能与转录因子 NF-κB的激活有关
As a chemotactic cytokine, IL - 8 plays an important role in the inflammatory response. Its expression in endothelial cells is regulated by various cytokines. After incubation of cultured human umbilical vein endothelial cells with TNF-α for different time, the expression of IL-8mRNA in endothelial cells was detected by RT-PCR and the activation of NF-κB in endothelial cells was detected by immunocytochemical staining. The results showed that: (1) IL - 8 expression in endothelial cells incubated with TNF - α was very low, the expression of IL - 8 increased significantly after 1 h of TNF - α incubation and further increased at 3 h, (2) The immunocytochemical staining of NF-κB p65 in nuclei of endothelial cells that were not incubated with TND-α was negative. Immunocytochemical staining of NF-κB p65 in NF-α 1,3 h after incubation was negative Positive, weak positive after 6 h, negative after 9 h. It is suggested that TNF-α can induce endothelial cells to express IL - 8 and activate NF - κB in endothelial cells. The phase process of TNF - α is basically the same. The author believes that TNF-α-induced IL-8 expression in endothelial cells may be related to the activation of transcription factor NF-κB