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目的:观察凝血酶-抗凝血酶复合物(TAT)、肠脂肪酸结合蛋白(IFABP)在肠系膜上动脉栓塞(SMAE)模型犬外周血含量的变化,探讨SMAE的早期诊断指标。方法:自体血栓栓塞法建立SMAE动物模型,酶联免疫法检测犬外周血TAT、IFABP及D-二聚体(DD)含量,自动生化分析仪测乳酸脱氢酶(LDH)。结果:栓塞后30min,SMAE组TAT值、IFABP值均明显升高,与正常对照组比较,差异有统计学意义,但与假手术组比较,TAT值栓塞前1h差异无统计学意义,各时间点的IFABP值差异均有统计学意义。SMAE犬外周血DD值、LDH值也明显升高,但升高时间迟于TAT和IFABP。检验效能分析显示:联合TAT和IFABP检测可保持较高的诊断敏感度,并提高诊断特异性。结论:TAT、IFABP可能作为SMAE的早期诊断指标,但需将二者联合检测,以提高诊断特异性。
Objective: To observe the changes of peripheral blood levels of thrombin-antithrombin complex (TAT) and intestinal fatty acid binding protein (IFABP) in the model of superior mesenteric artery embolism (SMAE) in dogs and to explore the early diagnosis of SMAE. Methods: Animal models of SMAE were established by autologous thromboembolism. The levels of TAT, IFABP and D-dimer in peripheral blood of dogs were detected by enzyme-linked immunosorbent assay (ELISA) and lactate dehydrogenase (LDH) by automatic biochemical analyzer. Results: The values of TAT and IFABP in SMAE group were significantly increased at 30 min after embolization. Compared with the normal control group, the difference was statistically significant. However, there was no significant difference in the value of TAT between before and 1 h after embolization Point IFABP values were statistically significant differences. The values of DD and LDH in peripheral blood of SMAE dogs also increased obviously, but the rising time was later than that of TAT and IFABP. Test performance analysis showed that: combined with TAT and IFABP test can maintain a high diagnostic sensitivity and improve diagnostic specificity. Conclusion: TAT and IFABP may be used as early diagnostic indicators of SMAE, but they should be combined to improve the diagnostic specificity.