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目的:观察降钙素相关肽α(calcitonin-related polypeptide alpha,CALCA)的蛋白表达水平与宫颈病变病理进程的关系。方法:收集维吾尔族妇女宫颈炎、低度鳞状上皮内瘤变(LSIL)、高度鳞状上皮内瘤变(HSIL)和宫颈鳞癌(CSCC)患者的石蜡包埋组织标本共104例,采用免疫组织化学方法检测CALCA蛋白的表达水平。结果:CALCA蛋白在大部分宫颈炎患者的宫颈上皮细胞中强表达,但是随着宫颈上皮内瘤变和宫颈癌的发生,其表达水平呈现从强表达到弱表达或表达缺失的趋势改变,且与宫颈病变病理进程呈负相关(r=-0.361,P<0.01),其中宫颈炎、LSIL、HSIL和CSCC组患者的CALCA蛋白表达缺失率分别为45.5%(15/33)、75%(12/16)、80.8%(21/26)和82.8%(24/29),C ALCA蛋白表达缺失率在HSIL及CSCC与宫颈炎间的差异具有统计学意义(P<0.01),而在LSIL与宫颈炎间的差异无统计学意义(P>0.05)。结论:CALCA蛋白表达缺失与维吾尔族妇女宫颈癌前病变及癌症的发生有一定的关系。
Objective: To investigate the relationship between the protein expression of calcitonin-related polypeptide alpha (CALCA) and the pathological process of cervical lesions. Methods: A total of 104 paraffin-embedded tissues from Uighur women with cervicitis, low-grade squamous intraepithelial neoplasia (LSIL), high-grade squamous intraepithelial neoplasia (HSIL) and cervical squamous cell carcinoma (CSCC) Immunohistochemistry was used to detect the expression of CALCA protein. Results: CALCA protein was strongly expressed in cervical epithelial cells of most patients with cervicitis, but with the development of cervical intraepithelial neoplasia and cervical cancer, the expression level of CALCA protein changed from strong expression to weak expression or lack of expression (R = -0.361, P <0.01). The deletion rates of CALCA protein in cervicitis, LSIL, HSIL and CSCC group were 45.5% (15/33) and 75% (12) respectively /16), 80.8% (21/26) and 82.8% (24/29), respectively. There was a significant difference in the loss of C ALCA protein expression between HSIL, CSCC and cervicitis (P <0.01) There was no significant difference between cervicitis (P> 0.05). Conclusion: The loss of CALCA protein expression is related to the occurrence of cervical precancerous lesions and cancers in Uighur women.