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谷子源于中国,遗传多样性丰富,近年来,科研人员培育出来了许多新品种,但是,可选用的目标种质资源不多,育种形势不乐观。ISSR荧光标记毛细管电泳法是一种以DNA测序为平台的检测方法,拥有更高的自动化、准确性等一些优点。本实验利用ISSR荧光标记毛细管电泳技术对30个优异的谷子品种进行了遗传多样性分析。ISSR分析结果也表明参试的谷子品种遗传变异丰富,8条ISSR引物扩增出120多条多态性条带,遗传相似系数的变化范围为0.89~0.93。数据处理后聚类结果发现一些地理来源不一致的品种也被聚集在一起,这种情况的出现或许是因为人类的行为所造成的,使得谷子品种间体现出了近亲缘的关系。本实验可以为谷子品种的分类鉴定、谷子优异种质的科学利用及分子标记辅助育种提供理论依据。
Millet originated from China and is rich in genetic diversity. In recent years, many new varieties have been cultivated by scientific researchers. However, the target germplasm resources available for selection are few, and the breeding situation is not optimistic. ISSR fluorescence-labeled capillary electrophoresis is a DNA sequencing-based detection method, with higher automation, accuracy and some other advantages. In this study, genetic diversity of 30 excellent millet cultivars was analyzed using ISSR fluorescence-labeled capillary electrophoresis. ISSR analysis also showed that genetic diversity of the tested millet varieties was rich. Eight ISSR primers amplified more than 120 polymorphic bands, and the genetic similarity coefficient ranged from 0.89 to 0.93. After data processing, the clustering results showed that some varieties with different geographic sources were also brought together. This situation may be caused by human behavior, which makes the millet varieties show close kinship. This experiment can provide theoretical basis for classification and identification of millet varieties, scientific utilization of millet excellent germplasm and molecular marker-assisted breeding.