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目的：制备含有２．０拷贝乙型肝炎病毒（ＨＢＶ，ａｄｒ亚型）基因组的转基因小鼠，为ＨＢＶ相关医学问题的研究准备实验动物模型。方法：应用受精卵原核显微注射的方法，产生ＨＢＶ转基因小鼠。用ＰＣＲ，Ｓｏｕｔｈｅｒｎ－ｂｌｏｔｉｎｇ杂交、放射免疫、免疫组织化学以及亚显微结构分析等方法，研究外源基因在转基因小鼠体内的整合、表达以及复制。应用常规病理切片及血清生物化学方法分析转基因小鼠的病理学改变。结果：得到了４只整合有ＨＢＶ基因组的建立者小鼠，并证明ＨＢＶ基因在转基因小鼠体内可以稳定遗传，在肝、肾组织内可特异性表达、复制，并在肝细胞内发现了Ｄａｎｅ颗粒。转基因小鼠的肝、肾组织未见明显的病理学改变。结论：获得了ＨＢＶ的转基因小鼠，其病毒基因可以表达，并有病毒颗粒形成。该小鼠对于ＨＢＶ的各个基因产物是免疫耐受的，其基本生物学特性与人类的ＨＢＶ携带者特征相似。
Objective: To prepare transgenic mice containing 2.0 copies of hepatitis B virus (HBV, adr subtype) genome and prepare experimental animal models for the study of HBV related medical problems. Methods: Using fertilized egg prokaryotic microinjection method to produce HBV transgenic mice. The integration, expression and replication of exogenous genes in transgenic mice were studied by PCR, Southern blotting, radioimmunoassay, immunohistochemistry and sub-microstructure analysis. The pathological changes of transgenic mice were analyzed by routine pathology and serum biochemical methods. Results: Four founder mice with HBV genome were obtained and proved that HBV gene can be stably inherited in transgenic mice and specifically expressed and replicated in liver and kidney. Dane was found in hepatocytes Granules. There was no obvious pathological change in liver and kidney of transgenic mice. CONCLUSION: The transgenic mice obtained with HBV can express their viral genes and form viral particles. This mouse is immunologically tolerant to the individual gene products of HBV and has basic biological characteristics similar to those of human carriers of HBV.