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目的:观察二黄汤对哮喘模型大鼠肺功能、气道壁结构及血清、BALF中尾加压素-Ⅱ(U-Ⅱ)含量的影响,探讨二黄汤干预哮喘的作用机理。方法:将32只健康SD大鼠随机分为正常对照组、模型组、布地奈德组、二黄汤组,每组8只,采用OVA致敏加激发法复制大鼠哮喘模型,分别对各组大鼠给予相应治疗后,对大鼠肺功能进行检测,HE染色观察大鼠气道壁病理形态学改变并测量其各层厚度,用ELISA法检测大鼠血清、BALF中U-Ⅱ浓度。结果:与正常对照组比较,模型组肺功能显著下降(P<0.001),气道上皮严重损伤,气道壁显著增厚(P<0.001),血清、BALF中U-Ⅱ含量显著升高(P<0.001)。与模型组比较,各治疗组肺功能均有所改善(P<0.01或P<0.001),气道上皮损伤减轻,气道壁厚度降低(P<0.001),血清、BALF中U-Ⅱ含量降低(P<0.001)。结论:二黄汤能明显改善哮喘模型大鼠症状,其作用机理可能与降低U-Ⅱ升高有关。
Objective: To observe the effects of Erhuangtang on the lung function, airway wall structure and the content of urotensin-Ⅱ (U-Ⅱ) in serum and BALF in asthmatic rats, and to explore the mechanism of Erhuang Decoction on asthma. Methods: Thirty-two healthy SD rats were randomly divided into normal control group, model group, budesonide group and Erhuang Decoction group, with 8 rats in each group. The asthma model was induced by OVA sensitization plus excitement, Rats were given the appropriate treatment, the lung function was detected, the pathological changes of the airway wall were observed by HE staining and the thickness of each layer was measured. The concentration of U-Ⅱ in the serum and BALF of the rats was detected by ELISA. Results: Compared with the normal control group, the lung function of the model group was significantly decreased (P <0.001), the airway epithelium was severely damaged, the airway wall was significantly thicker (P <0.001), and the content of U-Ⅱ in serum and BALF was significantly increased P <0.001). Compared with the model group, pulmonary function was improved in each treatment group (P <0.01 or P <0.001), airway epithelial lesion lessened, airway wall thickness decreased (P <0.001), serum and BALF U-Ⅱlevel decreased (P <0.001). Conclusion: Erhuang Decoction can significantly improve the symptoms of asthma in rats, and its mechanism may be related to the decrease of U-Ⅱ.