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目的分析异常黑胆质与载脂蛋白E基因多态性的关联。方法按照《维吾尔医诊断学》标准诊断喀什地区维吾尔族居民异常黑胆质组515例(异常黑胆质组),诊断正常体液类型308例作为正常体液组,采用高分辨熔解取合酶链反应(HMR-PCR方)法对ApoE基因进行基因分型,分析ApoE基因rs405509、rs769450位点多态性,比较两组基因型和等位基因的分布频率。结果异常黑胆质组和正常体液组rs405509位点主要基因型为AC(47.7%和51.0%),主要等位基因为A(54.6%和60.1%);rs769450位点主要基因型为GG(48.3%和49.7%),主要等位基因为G(69.4%和72.1%);异常黑胆质组ApoE基因rs405509位点CC基因型和C等位基因频率高于正常体液组(P<0.05),两组rs769450和rs405509位点A G*单倍比较,差异有统计学意义(P<0.01)。结论 rs405509位点多态性、rs769450和rs405509位点A G*单倍与异常黑胆质有关联。
Objective To analyze the association between abnormal savda and apolipoprotein E gene polymorphism. Methods According to the standard of “Uighur medical diagnosis and diagnosis”, 515 cases of abnormal savda group (abnormal savda group) of Uighur residents in Kashgar region were diagnosed, and 308 cases of normal body fluid type as normal body fluid group were diagnosed. High resolution melting and enzyme linked reaction (HMR-PCR) method was used to genotype the ApoE gene. The rs405509 and rs769450 polymorphisms of the ApoE gene were analyzed. The frequencies of genotypes and alleles in the two groups were compared. Results The major genotypes of rs405509 in the savda group and normal body fluid group were AC (47.7% and 51.0%), the major alleles were A (54.6% and 60.1%), and the major genotypes of rs769450 were GG (48.3 % And 49.7% respectively). The major alleles were G (69.4% and 72.1%). The frequency of CC genotype and C allele in rs405509 of ApoE gene in abnormal savda group were higher than those in normal body fluid group (P <0.05) Two groups of rs769450 and rs405509 loci AG * haplogroup comparison, the difference was statistically significant (P <0.01). Conclusion rs405509 polymorphism, rs769450 and rs405509 A G * haplotype associated with abnormal savda.