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目的 :采用持续经皮贴硝酸甘油 ( 0 .4mg/h) 72小时建立大白兔耐药模型 ,用鲁米诺化学发光技术测量血管壁超氧阴离子 (·O-2 )水平 ,探讨·O-2 水平与硝酸酯耐药性的关系。方法 :动物随机分为硝酸甘油组和对照组 ,贴膜 72小时后获取胸主动脉 ,观察血管环对不同浓度硝酸甘油的舒张反应和测量血管组织·O-2 、丙二醛 (MDA)水平和超氧化物歧化酶 (SOD)活性。结果 :硝酸甘油组血管环对硝酸甘油舒张反应明显减弱 (最大舒张反应 58.76±1 2 .96%vs94.68± 3.2 3% ,P <0 .0 5) ,硝酸甘油组血管组织·O-2 水平较对照组显著增加 ( 2 31 7.38± 889.0 3vs50 8±1 70 .94cps/g .ml,P <0 .0 5) ,而SOD活性和MDA水平无显著改变 (P >0 .0 5)。结论 :持续经皮贴硝酸甘油 72小时血管环产生了明显耐药 ,耐药的产生可能与血管壁超氧阴离子增加有关
OBJECTIVE: To establish a model of drug resistance in rabbits by continuous transdermal administration of nitroglycerin (0. 4mg / h) for 72 hours and measure the level of superoxide anion (· O-2) by luminol chemiluminescence technique. 2 levels and nitrate resistance relationship. Methods: The animals were randomly divided into nitroglycerin group and control group. After 72 hours, the thoracic aorta was obtained and the vasodilatory responses of vascular rings to different concentrations of nitroglycerin and the levels of serum O-2, malondialdehyde (MDA) and Superoxide dismutase (SOD) activity. Results: The vasoconstriction of nitroglycerin group in nitroglycerin group was significantly weakened (the maximal diastolic reaction was 58.76 ± 122.96% vs94.68 ± 3.2 3%, P <0.05) (2 31 7.38 ± 889.0 3 vs 50 8 ± 1 70.94 cps / g · ml, P <0.05), while the activity of SOD and MDA did not change significantly (P> 0.05). CONCLUSION: Sustained transdermal nitration of nitroglycerin produces a significant resistance to 72-hour vascular rings, which may be related to the increase of superoxide anion in vascular wall