论文部分内容阅读
目的：观察高压氧对急性缺血再灌流免下丘脑β-内啡肽的影响、方法：将实验白兔随机分成高压氧治疗组和对照组，双颈总动脉夹闭加低血压制成急性不完全性脑缺血动物模型。在再灌流６０ｍｉｎ期间，高压氧治疗组在０．２５ＭＰａ下吸纯氧，对照组在常压下吸空气。取免下丘脑，用光镜免疫组化技术，观察β－内啡肽的变化。结果：高压氧治疗组神经细胞完整性较好，细胞内β－内啡肽多，细胞间质β－内啡肽少。而对照组大部分神经细胞浊肿，β－内啡肽细胞内少于细胞间质；彩色图像分析结果显示，高压氧治疗组与对照组在内啡肽灰度、目标数和目标面积方面有非常显著的统计学差异（Ｐ＜０．０１）。结论：高压氧治疗有减少β－内啡肽从神经细胞内逸出的保护作用，减轻β－内啡肽逸出造成脑的继发性损伤。
OBJECTIVE: To observe the effect of hyperbaric oxygen on hypothalamic β-endorphin in acute ischemic reperfusion in rabbits.Methods: The experimental rabbits were randomly divided into hyperbaric oxygen therapy group and control group, bilateral common carotid artery occlusion plus hypotension was made acute Incomplete cerebral ischemia in animal models. During the 60min reperfusion, hyperbaric oxygen therapy group inhaled pure oxygen at 0.25MPa and control group inhaled air at atmospheric pressure. Remove the hypothalamus, using light microscopy immunohistochemistry to observe the changes of β-endorphin. Results: The integrity of neurons in hyperbaric oxygen therapy group was better, with more β-endorphin and fewer interstitial β-endorphin in the cells. However, most of the neuronal cells in the control group were cloudy and cytoplasm were less in the β-endorphin cells. The results of color image analysis showed that in the hyperbaric oxygen treatment group and the control group, the endorphin gray level, target number and target area Very significant statistical difference (P <0.01). CONCLUSION: Hyperbaric oxygen therapy can reduce the protective effect of β-endorphin from escaping inside neurons and reduce secondary brain injury caused by the release of β-endorphin.